Bacteriophage MB78 DNA synthesis is specifically inhibited by the chelating agent ethylene diamine tetraacetic acid
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چکیده
منابع مشابه
An automated method for serum calcium utilizing ethylene diamine tetraacetic acid.
A FUNDAMENTAL DIFFICULTY in estimating calcium in serum by photometry arises from the fact that the normal range is narrow relative to the concentration of calcium. In the cases of sodium and chloride which also possess narrow normal ranges, the automation problem has been approached in two different ways. For sodium the Technicon Flame Photometer incorporates an electrical range expander. In t...
متن کاملInhibition of DNA synthesis in animal cells by ethylene diamine tetraacetate, and its reversal by zinc.
During an investigation of the role of ions in growth regulation, it was found that ethylene diamine tetraacetate (EDTA) inhibits DNA synthesis about 10-fold in cultures of chick embryo cells, while ethylene glycol bis(beta-amino ethyl ether)-N,N'-tetraacetate has no effect. RNA synthesis is only slightly inhibited by EDTA, and protein synthesis is unaffected. EDTA is inhibitory to DNA synthesi...
متن کاملFunction of the classical and alternate pathways of human complement in serum treated with ethylene glycol tetraacetic acid and MgCl2-ethylene glycol tetraacetic acid.
An immunochemical and functional analysis of the classical and alternate complement pathways in human serum was performed in the presence of 10 mM ethylene glycol tetraacetic acid (EGTA) and MgCl(2)-EGTA (MgEGTA), chelating agents which have been recently utilized as a means of distinguishing between these two complement pathways. Total hemolytic activity, integrity of the C1 complex, hemolytic...
متن کاملEffect of citric acid chelating agent on morphology of ZnO particles prepared by hydrothermal method
Abstract Zinc oxide is one of the most widely used and important materials in today's science and industry. In this study, ZnO nanoparticles were synthesized by hydrothermal method. Zinc acetate, citric acid and deionized water was respectively used as a source of zinc ion, solvent and chelating agent. In this study, the effect of citric acid concentration as an additive was studied on morphol...
متن کاملIsolation and Characterization of Thermophilic Alkaline Proteases Resistant to Sodium Dodecyl Sulfate and Ethylene Diamine Tetraacetic Acid from Bacillus sp. GUS1
Thermophilic Bacillus sp. GUS1, isolated from a soil sample obtained from citrus garden, produced at least three proteases as detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and zymogram analysis. The enzymes were stable in the alkaline pH range (8.0-12.0), with the optimum temperature and pH range of the proteases being 70ºC and 6.0-12.0, respectively. All th...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1987
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1987.tb02253.x